Baird-Parker-Agar (BPA)
| Shorthand symbol: | BPA |
| Item number: | 40-1104 |
| Format: | Petri Dish, 90mm |
| Colour: | Beige, opaque |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 3 Months |
| pH: | 6.8 ± 0.2 at 25°C |
Baird-Parker-Agar is designed for the isolation and differentiation of Staphylococcus aureus in food (§ 64 LFGB) and pharmaceutical products.
| in g per 1l Medium | |
|---|---|
| Casein peptone, pancreatically digested | 10 |
| Meat extract | 5 |
| Yeast extract | 1 |
| Lithium chloride | 5 |
| Glycine | 12 |
| Sodium pyruvate | 10 |
| Egg yolk (in ml) | 10 |
| Sodium chloride solution (in ml) | 40 |
| Potassium tellurite | 0.01 |
| Agar | 12 |
The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.
Productivity
Incubation conditions: 24±2 to 48±2 hours at 37±1°C; Inoculum concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 25923/WDCM 00034 | 50–130 % | Black or grey colonies with clearing zone (egg yolk hydrolysis reaction) |
Selectivity
Incubation conditions: 48±2 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | Complete inhibition | - |
Specificity
Incubation conditions: 24±2 to 48±2 hours at 37±1 °C; Inoculum concentration: 1.000–10.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus saprophyticus | ATCC 15305/WDCM 00159 | Good growth | Black or grey colonies without clearing zone (egg yolk hydrolysis reaction) | |
| Staphylococcus Epidermidis | ATCC 12228/WDCM 00036 | Good growth | Black or grey colonies without clearing zone (egg yolk hydrolysis reaction) |
Mikrobial Contamination
Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C
| Specification |
|---|
| No microbial contamination |