Baird-Parker-Agar (BPA)


Shorthand symbol: BPA
Item number: 40-1104
Format: Petri Dish, 90mm
Colour: Beige, opaque
Storage conditions: Dry, in closed bag, at 4 – 10°C.
Shelf-life: 3 Months
pH: 6.8 ± 0.2 at 25°C
1104 baird parker agar bpa xebios
Intented use and fields of application

Baird-Parker-Agar is designed for the isolation and differentiation of Staphylococcus aureus in foods (§ 64 LFGB) and pharmaceutical products.


Typical composition in g/l

in g per 1 litre of Nutrient medium
Pancreatic-digest of Casein peptone10.0
Beef Extract5.0
Yeast Extract1.0
Lithium Chloride5.0
Glycine12.0
Sodium pyruvate10.0
Egg yolk (in ml)10.0
Sodium Chloride solution(in ml)40.0
Potassium Tellurite0.01
Agar20.0


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.

 
Productivity
Incubation conditions: 24±2 to 48±2 hours at 37±1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 0003450–130 %Black or grey colonies with clearing zone (egg yolk hydrolysis reaction)


Selectivity

Incubation conditions: 48±2 hours at 37±1°C; Inoculum concentration: 10,000–1,000,000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-

Specificity
Incubation conditions: 24±2 to 48±2 hours at 37±1 °C; Inoculum concentration: 1,000–10,000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus saprophyticusATCC 15305/WDCM 00159Good growthBlack or grey colonies without clearing zone (egg yolk hydrolysis reaction)
Staphylococcus EpidermidisATCC 12228/WDCM 00036Good growthBlack or grey colonies without clearing zone (egg yolk hydrolysis reaction)


Mikrobial Contamination

Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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