Baird-Parker-Agar (BPA)


Shorthand symbol: BPA
Item number: 40-1104
Format: Petri Dish, 90mm
Colour: Beige, opaque
Storage conditions: Dry, in closed bag at 4 – 10°C.
Shelf-life: 3 Months
pH: 6.8 ± 0.2 at 25°C
1104 baird parker agar bpa xebios
Intented use and fields of application

Baird-Parker-Agar is designed for the isolation and differentiation of Staphylococcus aureus in foods (§ 64 LFGB) and pharmaceutical products.


Typical composition in g/l

in g per 1l Medium
Casein peptone, pancreatically digested10
Meat extract
5
yeast extract
1
Lithium chloride5
Glycine12
sodium pyruvate10
Egg yolk (in ml)10
Sodium chloride solution (in ml)40
potassium tellurite0.01
Agar12
*Adjusted as required to meet performance standards


Microbiological quality control

The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.

 
Productivity
Incubation conditions: 24±2 to 48±2 hours at 37±1°C; Inoculum concentration: 80–120 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus aureusATCC 25923/WDCM 0003450–130 %Black or grey colonies with clearing zone (egg yolk hydrolysis reaction)


Selectivity

Incubation conditions: 48±2 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU

OrganismType StrainSpecificationColony morphology
Escherichia coliATCC 8739/WDCM 00012Complete inhibition-

Specificity
Incubation conditions: 24±2 to 48±2 hours at 37±1 °C; Inoculum concentration: 1.000–10.000 CFU

OrganismType StrainSpecificationColony morphology
Staphylococcus saprophyticusATCC 15305/WDCM 00159Good growthBlack or grey colonies without clearing zone (egg yolk hydrolysis reaction)
Staphylococcus EpidermidisATCC 12228/WDCM 00036Good growthBlack or grey colonies without clearing zone (egg yolk hydrolysis reaction)


Mikrobial Contamination

Incubation conditions: 5–7 days, 20–25°C and 5–7 days, 30–35°C

Specification
No microbial contamination


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