Bacillus-Cereus-Agar (PEMBA)
| Shorthand symbol: | PEMBA |
| Item number: | 40-1245 |
| Format: | Petri dish, 90mm |
| Colour: | Yellowish, evenly cloudy |
| Storage conditions: | Dry, closed at 4-10 ° C |
| Shelf-life: | 3 Months |
| pH: | 7.2 ± 0.2 at 25°C |
Selective nutrient medium for isolation, identification and bacterial count of Bacillus Cereus in food.
| in g per 1l Medium | |
|---|---|
| Peptone (in g) | 1 |
| Mannitol (in g) | 10 |
| Sodium pyruvate (in g) | 10 |
| Sodium chloride (in g) | 2 |
| Disodium hydrogen phosphate(in g) | 2.5 |
| Potassium dihydrogen phosphate (in g) | 0.25 |
| Magnesium sulphate (in g) | 0.05 |
| Bromothymol blue (in g) | 0.12 |
| Polymyxin B Sulphate (in mg) | 13 |
| Egg yolk (in ml) | 10 |
| Sodium chloride solution (in ml) | 40 |
| Agar | 14 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 21 ± 3 to 44 ± 4 hours at 37 ± 1 ° C; Inoculum Concentration: 80 - 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Bacillus cereus | ATCC 11778 / WDCM 00001 | 50 – 130 % | Turquoise blue colonies with precipitate courtyard |
Selectivity
Incubation conditions: 44 ± 4 hours at 37 ± 1 ° C; Inoculum Concentration: 10.000 - 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - |
Specificity
Incubation conditions: 44 ± 4 hours at 37 ± 1 ° C; Inoculum Concentration: 10.00 - 10.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Bacillus subtilis | ATCC 6633 / WDCM 00003 | Normal growth | White colonies without precipitate |
Microbial Contamination
Incubation conditions: 5 – 7 Tage at 20 – 25 °C and 5 – 7 Tage at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |