Azid-Glucose-Bouillon nach Rothe
| Shorthand symbol: | AGB |
| Item number: | 60-1103 |
| Format: | Vial, 9ml |
| Colour: | Clear, gold-yellowish |
| Storage conditions: | Dry, closed at 15-22°C |
| Shelf-life: | 2 Months |
| pH: | 7.2 ± 0.2 at 25°C |
Azide-glucose broth is very nutritious and offers very good growth conditions. The sodium azide contained in it serves as an inhibitor of gram-negative bacteria.Azide Glucose Broth is used to study water and waste water for fecal contamination. The presence of enterococci is considered an indicator of contamination, as they have a relatively high tolerance to chlorine compared to Escherichia coli. The contained sodium azide suppresses the gram-negative accompanying flora of the enterococci.
| in g per 1l Nutrient medium | |
|---|---|
| Peptone | 19.8 |
| Glucose | 7.5 |
| Sodium chloride | 7.5 |
| Sodium azide | 0.2 |
| *Adjusted as required to meet performance standards |
The microbial performance test is carried out in conformity with the requirements of DIN EN ISO 11133.
Productivity
Incubation conditions: 44 ± 4 hours at 36 ± 2 °C; Inoculum concentration: 80 – 120 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Significant, cloudy | Vaccination and Enterococcus-selective agar according to Slanetz-Bartley | |
| Enterococcus faecium | ATCC 6057 / WDCM 00177 | Significant. cloudy | Vaccination and Enterococcus-selective agar according to Slanetz-Bartley |
Selectivity
Incubation conditions: 44 ± 4 hours 37 ± 1 °C; Inoculum concentration: 10.000 – 1.000.000 CFU
| Organism | Test strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 6538 / WDCM 00032 | Complete inhibition | - | |
| Escherichia coli | ATCC 8739 / WDCM 00012 | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5 – 7 days at 20 – 25 °C and 5 – 7 days at 30 – 35 °C
| Specification |
|---|
| No microbial contamination |