Streptokokken-Selektivagar (SESA)
| Shorthand symbol: | SESA |
| Item number: | 40-1507 |
| Format: | Petri Dish, 90mm |
| Colour: | Blood-red, opaque |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 2 Months |
| pH: | 7.3 ± 0.2 at 25°C |
Streptococcus Selective Agar allows the isolation of Streptococci species and Enterococci species from clinical materials and foods.
| in g per 1l Medium | |
|---|---|
| Casein peptone | 15.0 |
| Soya peptone | 5.0 |
| Sodium chloride | 5.0 |
| Aesculin | 1.0 |
| Ammonium Iron (III) citrate | 0.5 |
| Colistine | 0.01 |
| Oxalic acid | 0.005 |
| Neomycin sulphate | 0.02 |
| Sheep blood (in ml) | 50.0 |
| Agar | 17.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the following specification:
Productivity
Incubation Conditions: 24 ± 4 hours at 36 ± 2 °C; Inoculum concentration of Target Organisms: 80 – 120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Streptococcus pyogenes | ATCC 12344 | 50–130 % | Small, grey colonies with significant hydrolysis | |
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | 50–130 % | Small, grey colonies with blackening of the agar media around the colonies |
Selectivity
Incubation conditions: 24±4 hours at 36±2°C; Inoculum concentration: Target organisms: 10.000–1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | Complete or partial inhibition | - | |
| Staphylococcus aureus | ATCC 6538/WDCM 00032 | Complete or partial inhibition | - |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35 °C
| Specification |
|---|
| No microbial contamination |