Sabouraud-Dextrose-Agar nach harm. EP/USP/JP (SAB)
| Shorthand symbol: | SAB |
| Item number: | 40-1264 |
| Format: | Petri Dish, 90mm |
| Colour: | Yellowish |
| Storage conditions: | Dry, in closed bag at 15 - 22°C |
| Shelf-life: | 5 Months |
| pH: | 5.6 ± 0.2 at 25°C |
Universal Nutrient medium for the cultivation and enumeration of moulds and yeasts and dematophytes. Sabouraud Dextrose Agar contains 4% dextrose, which promotes the growth of these microorganisms. The low pH promotes spore and pigment formation of yeast and fungi, and inhibits the growth of bacteria. It therefore is a selective culture.
This Nutrient medium complies with the requirements of the methods of the EP/USP/JP. The use of Sabouraud Dextrose Agar medium according to the EP/USP/JP is recommended for the full analysis of Candida albicans in non-sterile pharmaceutical products.
| in g per 1 litre Nutrient medium | |
|---|---|
| Pancreatic-digest of Casein Peptone | 5.0 |
| Pancreatic-digest of Beef Extract Peptone | 5.0 |
| Dextrose | 40.0 |
| Agar | 15.0 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements BS EN ISO 11133:2014 and Pharm. Eur. (Microbiological Examination of non-sterile products acording to Chapter 2.6.13).
Productivity
Incubation conditions: 2–3 days at 30–35°C; Inoculum concentration: 10–100 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Candida albicans | ATCC 10231/WDCM 00054 | 50–200 % | Whitish, dry colonies | |
| Aspergillus brasiliensis | ATCC 16404/WDCM 00053 | 50–200 % | Brown, black conidia on mycelium | |
| Candida albicans (3–5 days, 20–25°C) | ATCC 10231/WDCM 00054 | 50–200 % | Whitish, dry colonies | |
| Aspergillus brasiliensis (3 – 5 days, 20 – 25 °C) | ATCC 16404/WDCM 00053 | 50–200 % | Brown, black conidia on mycelium |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
| Specification |
|---|
| No microbial contamination |