Rappaport-Vassiliadis-Salm.-Anr.bouillon (RVS) (RVS)
| Shorthand symbol: | RVS |
| Item number: | 60-1262 |
| Format: | Tube, 9ml |
| Colour: | turquoise, transparent |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 2 Months |
| pH: | 5.2 ± 0.2 bei 25°C |
The RVS Broth (Rappaport-Vassiliadis Salmonella Enrichment Broth according to the harmonised EP/USP/JP) is for the selective enrichment of Salmonella. This Medium complies with the recommendations of the harmonised EP/USP/JP (2006).
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Soya Peptone | 4.5 |
| Magnesium Chloride, anhydrous | 13.58 |
| Sodium Chloride | 8 |
| Dipotassium Phosphate | 0.4 |
| Potassium Dihydrogen Phosphate | 0.6 |
| Malachite Green | 0.036 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133:2014.
Productivity
Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 100 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica serotype Enteritidis + Escherichia coli + Pseudomonas aeroginosa | ATCC 13076/WDCM 00030 + ATCC 8739/WDCM 00012 + ATCC 27853/WDCM 00025 | > 10 colonies from XLD-Agar | Colonies with a black centre and a lightly transparent reddish zone due to the colour change of the medium(XLD) |
Selectivity
Incubation Conditions: 24±3 Hours at 41.5±1°C; Inoculum Concentration: 10,000 - 1,000,000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | < 100 Colonies in TSA | - | |
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | < 10 Colonies in TSA | - |
Microbial Contamination
Incubation Conditions: 5–7 Days, 20–25°C and 5–7 Days, 30–35°C
| Specification |
|---|
| No microbial contaminatkion |