Kristallviolett-Neutralrot-Galle-Gluc.-A. VRBD-ISO (VRBD-ISO)
According to ISO 21528
| Shorthand symbol: | VRBD-ISO |
| Item number: | 40-1148 |
| Format: | Petri Dish, 90mm |
| Colour: | Violet, transparent |
| Storage conditions: | Dry, in closed bag at 4 – 10°C. |
| Shelf-life: | 6 Months |
| pH: | 7.4 ± 0.2 at 25°C |
Violet Red Bile Dextrose Agar (VRBD) medium is for the detection and enumeration of bile-tolerant gram negative bacteria from foods such as milk and dairy products, egg products and animal carcasses. Moreover VRBD Agar is used for the detection of Enterobacterieceae in meat and dairy products.
| in g per 1 Litre nutrient medium | |
|---|---|
| Yeast Extract | 3 |
| Pancreatic-digest of gelatine peptone | 7 |
| Bile salts | 1.5 |
| Sodium chloride | 5 |
| Glucose monohydrate | 10 |
| Neutral red | 0.03 |
| Crystal Violet | 0.002 |
| Agar | 15 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133:2014.
Productivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: Target Organisms: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Salmonella enterica ssp. Abony | NCTC 6017/WDCM 00029 | 50–130 % | Pinkish red colonies with no odour or precipitate | |
| Escherichia coli | ATCC 8739/WDCM 00012 | 50–130 % | Pinkish red colonies with no odour or precipitate |
Selectivity
Incubation conditions: 24±2 hours at 37±1°C; Inoculum concentration: 10.000–1.000.000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Enterococcus faecalis | ATCC 19433/WDCM 00009 | Complete inhibition | - |
Microbial Contamination
Incubation conditions: 5–7 days at 20–25°C and 5–7 days at 30–35°C
| Specification |
|---|
| No microbial contamination |