Gepuffertes Peptonwasser (PEP)
| Shorthand symbol: | PEP |
| Item number: | 60-1137 |
| Format: | Tube, 9ml |
| Colour: | Clear, yellowish coloured |
| Storage conditions: | Dry, in closed bag, at 15-22°C |
| Shelf-life: | 6 Months |
| pH: | 7.0 ± 0.2 bei 25°C |
For enrichment of Salmonella from milk and dairy products and other microorganisms from other foods and from environmental samples.
| in g per 1 Litre of Nutrient medium | |
|---|---|
| Casein Peptone | 10 |
| Sodium Chloride | 5 |
| Disodium Hydrogen Phosphate | 3.5 |
| Potassium Hydrogen Phosphate | 1.5 |
The Microbiological Performance Test is carried out in accordance with the requirements of DIN EN ISO 11133.
Produktivität
Incubation conditions: 18 ± 2 Hours at 36 ± 2 °C; Inoculum Concentration: 80 – 120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 8739/WDCM 00012 | Very cloudy, turbid | - | |
| Salmonella enterica serovar Enteritidis | ATCC 13076/WDCM 00030 | Very cloudy, turbid | - |
Dilution
Incubation conditions: 45–60 Minutes at 22.5± 22,5 °C; Inoculum Concentration: 80-120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Staphylococcus aureus | ATCC 25923/WDCM 00034 | - | - | |
| Listeria monocytogenes | ATCC 13932/WDCM 00021 | - | - |
Microbial Contamination
Incubation conditions: 5–7 Days at 20–25°C and 5–7 Days at 30–35°C
| Specification |
|---|
| No microbial contamination |