Legionella-BCYE+AB-Agar (BCYE+AB)
According to ISO 11731
| Shorthand symbol: | BCYE+AB |
| Item number: | 40-1601 |
| Format: | Petri dish, 90mm |
| Colour: | Black, opaque |
| Storage conditions: | Keep away from light at constant temperature; at 4 – 25°C. |
| Shelf-life: | 3 Monate |
| pH: | 6.8 ± 0.2 at 25°C |
For the detection, isolation and enumeration of Legionella from water samples and clinical material.
| in g per 1l Medium | |
|---|---|
| Yeast Extract | 10 |
| Activated charcoal | 2 |
| ACES-Buffer | 10 |
| L-Cysteine | 0.4 |
| Iron (III) pyrophosphate | 0.25 |
| α-Ketoglutarate | 1 |
| Potassium hydroxide | 2.8 |
| Polymyxin B (in IE) | 80000 |
| Pimaricin | 0.07 |
| Cefazolin | 0.009 |
| Agar | 12 |
| *Adjusted as required to meet performance standards |
The Microbiological Performance Test is carried out in accordance with the requirements of ISO 11133.
Productivity
Incubation conditions: 2–5 days and 5–10 days at 36 ± 2 °C; Inoculation concentration: 80–120 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Legionella pneumophila | ATCC 33152 / WDCM 00107 | >50 % | White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance. | |
Legionella anisa (5-10 days) | ATCC 35392 / WDCM 00106 | >50 % | White-grey-blue-violet entire colonies with a smooth edge and a characteristic milky appearance. |
Selectivity
Incubation conditions: 3 days at 36 ± 2 °C; Inoculation concentration: 10,000-1,000,000 CFU
| Organism | Type Strain | Specification | Colony morphology | |
|---|---|---|---|---|
| Escherichia coli | ATCC 25922 / WDCM 00013 | Complete or partial inhibition. | - | |
| Enterococcus faecalis | ATCC 19433 / WDCM 00009 | Complete inhibition. | - |
Microbial Contamination
Incubation conditions: 48 ± 4h at 30 ± 1°C
| Specification |
|---|
| No microbial contamination |